Ribosome display: Cell-free protein display technology
نویسندگان
چکیده
منابع مشابه
Technique review Ribosome display: Cell-free protein display technology
Ribosome display is a cell-free system for the in vitro selection of proteins and peptides from large libraries. It uses the principle of coupling individual nascent proteins (phenotypes) to their corresponding mRNA (genotypes), through the formation of stable protein–ribosome–mRNA (PRM) complexes. This permits the simultaneous isolation of a functional nascent protein, through affinity for a l...
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In-vitro display technologies combine two important advantages for identifying and optimizing ligands by evolutionary strategies. First, by obviating the need to transform cells in order to generate and select libraries, they allow a much higher library diversity. Second, by including PCR as an integral step in the procedure, they make PCR-based mutagenesis strategies convenient. The resulting ...
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ژورنال
عنوان ژورنال: Briefings in Functional Genomics and Proteomics
سال: 2002
ISSN: 1473-9550,1477-4062
DOI: 10.1093/bfgp/1.2.204